Mucosal brush biopsy testing of the inferior turbinate to detect local, antigen-specific immunoglobulin E

This study demonstrates that Local Antibody Mucosal Brush Diagnostic (LAMB-Dx) testing from the nasal cavity is able to identify specific IgE (sIgE) to nine airborne allergens in individuals with allergic rhinitis (AR) who test positive on skin prick testing (SPT). In addition, sIgE are also detected on LAMB-Dx that are not detected on SPT, suggesting that the test could provide additional diagnostic information for the physician. This study establishes the feasibility of LAMB-Dx for allergy diagnosis.

Reisacher WR., Int Forum Allergy Rhinol. 2012 Jan-Feb;2(1):69-74.

Detecting Local immunoglobulin e from mucosal brush biopsy of the inferior turbinates using microarray analysis 

This study demonstrates that LAMB-Dx samples can be tested using microarray analysis technology to identify sIgE from nasal cavity tissue and the results agree with those obtained from serum testing using the ImmunoCAP method 98% of the time. This study illustrates that LAMB-Dx samples can be tested using multiple existing diagnostic platforms designed to detect sIgE from serum including microarray and ImmunoCAP.

Reisacher WR., Int Forum Allergy Rhinol. 2013 May:3(5):399-403.

Mucosal Brush Biopsy of the oral cavity to detect local, peanut-specific immunoglobulin E

This study demonstrates that the LAMB-Dx samples from the oral cavity can identify peanut-specific IgE in 100% of individuals who identified themselves as peanut allergic based on swelling and itching in the mouth and throat after consuming peanuts, while serum testing only identifies peanut-specific IgE in 50% of the same patient population. This work shows that LAMB-Dx can be used as an efficient diagnostic for food allergies as it correlates well with self-reported symptoms and can be easily and safely performed in individuals with severe food allergies.

Reisacher WR, Cohen JC. Int Forum Allergy Rhinol. 2013 Aug:3(8):636-41.

Prevalence of antigen-specific immunoglobulin e on mucosal brush biopsy of the inferior turbinates in patients with nonallergic rhinitis

This study demonstrates that 100% of patients presenting with non-allergic rhinitis (NAR), characterized by the presence of allergy symptoms but no sensitivities detected on skin and/or serum tested, who were tested with LAMB-Dx in the nasal cavity, have locally-present sIgE. In the sample population, 90% test positive to cockroaches, which is a common allergen in New York City, where testing was performed. Additionally, sensitivity to pollen by LAMB-Dx was associated with collection of the sample during the pollen season, suggesting the utility of this test as a seasonal allergy diagnostic.

Reisacher WR, Bremberg MG. Int Forum Allergy Rhinol. 2014 Apr;4(4)292-7.